KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | single turnover and burst kinetics, steady-state kinetics, recombinant His-tagged enzyme, overview | Deinococcus radiodurans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Deinococcus radiodurans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-proline + tRNAPro | Deinococcus radiodurans | - |
AMP + diphosphate + L-prolyl-tRNAPro | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Deinococcus radiodurans | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-proline + tRNAPro = AMP + diphosphate + L-prolyl-tRNAPro | class II synthetases are limited by a step prior to aminoacyl transfer | Deinococcus radiodurans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-proline + tRNAPro | - |
Deinococcus radiodurans | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | a two-step reaction | Deinococcus radiodurans | AMP + diphosphate + L-prolyl-tRNAPro | - |
? |
Synonyms | Comment | Organism |
---|---|---|
class II prolyl-tRNA synthetase | - |
Deinococcus radiodurans |
class II ProRS | - |
Deinococcus radiodurans |
Prolyl-tRNA synthetase | - |
Deinococcus radiodurans |
ProRS | - |
Deinococcus radiodurans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Deinococcus radiodurans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
tRNAPro | steady-state | Deinococcus radiodurans | |
9.8 | - |
tRNAPro | single turnover overall aminoacylation | Deinococcus radiodurans | |
10.8 | - |
tRNAPro | single turnover of the transfer step | Deinococcus radiodurans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Deinococcus radiodurans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Deinococcus radiodurans |